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KMID : 1161520180220040243
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Animal Cells and Systems
2018 Volume.22 No. 4 p.243 ~ p.252
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High-fat-diet impaired mitochondrial function of cumulus cells but improved the efficiency of parthenogenetic embryonic quality in mice
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Li Jingjing
Wang Shuang
Wang Bo
Wei Hao
Liu Xin
Hao Jun
Duan Yanping
Hua Jinlian
Zheng Xiaomin
Feng Xiuliang
Yan Xingrong
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Abstract
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Global human health has been compromised by high-fat diets. This study aimed to investigate the relationship between a high-fat diet and parthenogenetic embryo quality. Mice fed a high-fat or a normal diet was used as treated or control groups, respectively. Estradiol (E2), total cholesterol (TC) and total triglyceride (TG) were detected by Enzyme-Linked ImmunoSorbent Assay (ELISA). Cumulus-oocyte complexes (COCs) were collected from the mice in the treated and control groups. The ultrastructure of COCs, the expression level of genes involved in mitochondrial and nuclear functions in cumulus cells and oocytes quality were evaluated with transmission electron microscopy, real-time quantitative polymerase chain reaction (RT-PCR) and artificial parthenogenesis, respectively. The results showed that the efficiency of parthenogenetic embryonic development in vitro was significantly higher in the treated group than in the control group (p?
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KEYWORD
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Embryo, high-fat diet, mouse, oocyte, parthenogenesis
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